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Chem Impex International 01523 cas
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(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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Bio-Rad nylon 3 way stopcocks
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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European Directorate for the Quality of Medicines and HealthCare maximum storage duration
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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Cerilliant Corporation estrone
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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Chem Impex International hydroquinone
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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GraphPad Software Inc one and two-way anova multiple comparisons with graphpad prism 9.3.1
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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GraphPad Software Inc 3-way fixed factor anova graphpad prism 8.0
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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B. Braun three-way stopcocks
(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) <t>tigecycline</t> (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.
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Image Search Results


(A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) tigecycline (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.

Journal: bioRxiv

Article Title: Reactive Oxygen Detoxification Contributes to Mycobacterium abscessus Antibiotic Survival

doi: 10.1101/2024.10.13.618103

Figure Lengend Snippet: (A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) tigecycline (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.

Article Snippet: Antibiotics were used at the following concentrations: tigecycline (Chem-Impex) at 1.25ug/ml (8 fold above MIC, re-administered every 3 days), linezolid (Chem-Impex) at 2.5ug/ml (8 fold above MIC), rifampin (Sigma) at 32ug/ml (8 fold above MIC, re-administered every 6 days), isoniazid (Sigma) at 32ug/ml (8 fold above MIC, re-administered every 6 days), and ethambutol (Thermo) at 4ug/ml (8 fold above MIC, re-administered every 3 days).

Techniques: